Plant Transcription Factor Database
Previous version: v3.0
Arachis ipaensis
HSF Family
Species TF ID Description
Araip.2404GHSF family protein
Araip.24AK5HSF family protein
Araip.29KUEHSF family protein
Araip.2D8LNHSF family protein
Araip.3KN0AHSF family protein
Araip.3P0PJHSF family protein
Araip.4A18KHSF family protein
Araip.8V58JHSF family protein
Araip.90YM8HSF family protein
Araip.A5C77HSF family protein
Araip.A8GVJHSF family protein
Araip.B0RQSHSF family protein
Araip.DCD5QHSF family protein
Araip.FU4GLHSF family protein
Araip.G2FC5HSF family protein
Araip.K80UCHSF family protein
Araip.SMI4IHSF family protein
Araip.T98BRHSF family protein
Araip.U7I8RHSF family protein
Araip.UGV6FHSF family protein
Araip.Z1XB6HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645